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Home > Product > ELISA Kit > Rat Vascular Cell Adhesion Protein 1, VCAM-1 ELISA Kit

Rat Vascular Cell Adhesion Protein 1, VCAM-1 ELISA Kit

Rat Vascular Cell Adhesion Protein 1, VCAM-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.04ng/mL

Detection range: 6-1800ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.04ng/mL

Detection range: 6-1800ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Vascular cell adhesion protein 1

Code

E2318Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.04ng/mL

Detection range

6-1800ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

UniProt accession

MASS(Da)

81,246

GeneID

25361

Synonyms

CD antigen CD106;Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;VCAM1B

Gene names

Vcam1

Research area

Signal Transduction; Cancer

Target protein

VCAM-1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2318Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
960ng/mL 2.185 2.129 2.076
2.072
480ng/mL 1.231 1.252 1.2
1.273
240ng/mL 0.726 0.751 0.699
0.776
120ng/mL 0.437 0.416 0.363
0.394
60ng/mL 0.181 0.178 0.125
0.174
0ng/mL 0.044 0.052 0
0.061

Precision

We measured random samples of E2318Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 336.52 16.15 4.8
Intra-Assay 2 18 748.22 27.21 3.64
Intra-Assay 3 18 121.03 7.5 6.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2318Ra

    $320.00

  • 96T

    E2318Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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