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Home > Product > ELISA Kit > Human Serine Threonine-protein Kinase Pim-3, PIM3 ELISA Kit

Human Serine Threonine-protein Kinase Pim-3, PIM3 ELISA Kit

Human Serine Threonine-protein Kinase Pim-3, PIM3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 13.15ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 13.15ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

serine/threonine-protein kinase pim-3

Code

E2346Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

13.15ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

UniProt accession

MASS(Da)

35,891

GeneID

415116

Synonyms

PIM3;pim-3;Serine/threonine-protein kinase pim-3

Gene names

PIM3

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Target protein

PIM3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2346Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.28 2.206 2.154
2.131
1200ng/L 1.703 1.605 1.554
1.507
600ng/L 1.164 1.076 1.025
0.989
300ng/L 0.755 0.7 0.648
0.644
150ng/L 0.442 0.439 0.388
0.436
0ng/L 0.061 0.051 0
0.042

Precision

We measured random samples of E2346Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 953.16 46.63 4.89
Intra-Assay 2 18 258.19 14.28 5.53
Intra-Assay 3 18 563.81 43.51 7.72

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2346Hu

    $320.00

  • 96T

    E2346Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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