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Home > Product > ELISA Kit > Human Protein Wnt-5A, WNT5A ELISA Kit

Human Protein Wnt-5A, WNT5A ELISA Kit

Human Protein Wnt-5A, WNT5A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Wnt-5A

Code

E2352Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

UniProt accession

MASS(Da)

42,339

GeneID

7474

Synonyms

hWNT5A;Protein Wnt-5a;WNT5A

Gene names

WNT5A

Research area

Neuroscience; Stem Cells; Cancer

Target protein

WNT5A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2352Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.103 2.181 2.139
2.26
8ng/ml 1.25 1.32 1.278
1.39
4ng/ml 0.626 0.658 0.616
0.69
2ng/ml 0.45 0.439 0.397
0.429
1ng/ml 0.236 0.228 0.186
0.221
0ng/ml 0.032 0.042 0
0.053

Precision

We measured random samples of E2352Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.93 0.26 4.34
Intra-Assay 2 18 9.6 0.26 2.69
Intra-Assay 3 18 2.99 0.17 5.59

Protocols

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  • Size

    Code

    Price

  • 48T

    E2352Hu

    $320.00

  • 96T

    E2352Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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