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Home > Product > ELISA Kit > Mouse Protein Wnt-5B, WNT5B ELISA Kit

Mouse Protein Wnt-5B, WNT5B ELISA Kit

Mouse Protein Wnt-5B, WNT5B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Protein Wnt-5b

Code

E2402Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Protein Wnt-5b is a protein that in humans is encoded by the WNT5B gene. The WNT gene family consists of structurally-related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein showing 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates two transcript variants.

UniProt accession

MASS(Da)

40,343

GeneID

22419

Synonyms

AW545702;Protein Wnt-5b;Wnt-5;WNT5B;Wnt-5b

Gene names

Wnt5b

Research area

Stem Cells

Target protein

WNT5B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2402Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 1.908 1.962 1.922
2.017
6ng/mL 0.99 0.988 0.947
0.986
3ng/mL 0.485 0.5 0.46
0.515
1.5ng/mL 0.196 0.214 0.173
0.232
0.75ng/mL 0.126 0.12 0.079
0.113
0ng/mL 0.032 0.041 0
0.05

Precision

We measured random samples of E2402Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.24 0.16 3.02
Intra-Assay 2 18 9.27 0.27 2.91
Intra-Assay 3 18 3.98 0.22 5.62

Protocols

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  • Size

    Code

    Price

  • 48T

    E2402Mo

    $320.00

  • 96T

    E2402Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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