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Home > Product > ELISA Kit > Mouse Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12 ELISA Kit

Mouse Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12 ELISA Kit

Mouse Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.28ng/L

Detection range: 6-800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.28ng/L

Detection range: 6-800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tumor necrosis factor ligand superfamily member 12

Code

E2532Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.28ng/L

Detection range

6-800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).

UniProt accession

MASS(Da)

27,441

GeneID

21944

Synonyms

Apo3l;DR;Dr3l;Dr3lg;TNF-related weak inducer of apoptosis;TNFSF12;Tumor necrosis factor ligand superfamily member 12;Tumor necrosis factor ligand superfamily member 12, membrane form;Tumor necrosis factor ligand superfamily member 12, secreted form];Twea;Tweak

Gene names

Tnfsf12

Research area

Cell Biology; Cancer

Target protein

TNFSF12

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2532Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
960ng/L 1.918 1.966 1.927
2.014
480ng/L 0.912 0.945 0.906
0.978
240ng/L 0.435 0.498 0.459
0.561
120ng/L 0.192 0.237 0.198
0.282
60ng/L 0.093 0.104 0.065
0.115
0ng/L 0.037 0.039 0
0.041

Precision

We measured random samples of E2532Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 280.73 14.09 5.02
Intra-Assay 2 18 724.42 18.48 2.55
Intra-Assay 3 18 590.85 30.7 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2532Mo

    $320.00

  • 96T

    E2532Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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