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Home > Product > ELISA Kit > Human Structural Maintenance of Chromosomes Protein 3, SMC3 ELISA Kit

Human Structural Maintenance of Chromosomes Protein 3, SMC3 ELISA Kit

Human Structural Maintenance of Chromosomes Protein 3, SMC3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Structural Maintenance of Chromosomes Protein 3

Code

E3560Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

UniProt accession

MASS(Da)

141,542

GeneID

9126

Synonyms

Bamacan;Basement membrane-associated chondroitin proteoglycan;Chondroitin sulfate proteoglycan 6;Chromosome-associated polypeptide;hCAP;SMC 3;SMC protein 3;SMC3;SMC-3;Structural maintenance of chromosomes protein 3

Gene names

SMC3

Research area

Others

Target protein

SMC3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3560Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.415 2.364 2.322
2.313
4ng/ml 1.284 1.362 1.32
1.441
2ng/ml 0.725 0.731 0.689
0.737
1ng/ml 0.447 0.473 0.432
0.499
0.5ng/ml 0.305 0.299 0.257
0.293
0ng/ml 0.041 0.042 0
0.043

Precision

We measured random samples of E3560Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2 0.14 6.7
Intra-Assay 2 18 5.7 0.2 3.5
Intra-Assay 3 18 1.2 0.07 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3560Hu

    $320.00

  • 96T

    E3560Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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