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Home > Product > ELISA Kit > Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit

Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit

Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.58ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.58ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Vasodilator-stimulated phosphoprotein

Code

E2560Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.58ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity).

UniProt accession

MASS(Da)

39,667

GeneID

22323

Synonyms

Vasodilator-stimulated phosphoprotein;VASP

Gene names

Vasp

Research area

Signal Transduction; Neuroscience; Cell Biology; Cardiovascular; Metabolism

Target protein

VASP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2560Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.96 1.957 1.908
1.954
240ng/L 1.134 1.045 0.996
0.955
120ng/L 0.417 0.448 0.399
0.48
60ng/L 0.226 0.247 0.198
0.269
30ng/L 0.099 0.117 0.068
0.136
0ng/L 0.057 0.049 0
0.041

Precision

We measured random samples of E2560Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 111.03 5.14 4.63
Intra-Assay 2 18 323.18 9.72 3.01
Intra-Assay 3 18 218.72 7.89 3.61

Protocols

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  • Size

    Code

    Price

  • 48T

    E2560Mo

    $320.00

  • 96T

    E2560Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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