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Home > Product > ELISA Kit > Human Class E Basic Helix-Loop-Helix Protein 41, BHLHE41 ELISA Kit

Human Class E Basic Helix-Loop-Helix Protein 41, BHLHE41 ELISA Kit

Human Class E Basic Helix-Loop-Helix Protein 41, BHLHE41 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.58ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.58ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Class E Basic Helix-Loop-Helix Protein 41

Code

E3569Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.58ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed: 11278948, PubMed: 14672706, PubMed: 15193144, PubMed: 15560782, PubMed: 18411297, PubMed: 19786558, PubMed: 25083013). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed: 25083013). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).

UniProt accession

MASS(Da)

50,$498.00|$360.00

GeneID

79365

Synonyms

bHLHb3;BHLHE41;Class B basic helix-loop-helix protein 3;Class E basic helix-loop-helix protein 41;Differentially expressed in chondrocytes protein 2;Enhancer-of-split and hairy-related protein 1;hDEC2;SHARP-1

Gene names

BHLHE41

Research area

Others

Target protein

BHLHE41

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3569Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.478 2.443 2.398
2.409
320ng/L 1.491 1.52 1.474
1.548
160ng/L 0.718 0.825 0.78
0.933
80ng/L 0.398 0.436 0.39
0.473
40ng/L 0.217 0.203 0.157
0.188
0ng/L 0.03 0.046 0
0.062

Precision

We measured random samples of E3569Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 240.3 13.38 5.6
Intra-Assay 2 18 322.4 7.01 2.2
Intra-Assay 3 18 141.8 7.28 5.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E3569Hu

    $320.00

  • 96T

    E3569Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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