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Home > Product > ELISA Kit > Human Optineurin, OPTN ELISA Kit

Human Optineurin, OPTN ELISA Kit

Human Optineurin, OPTN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Optineurin

Code

E3579Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8 (PubMed: 27534431). Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation (PubMed: 27534431). Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation (PubMed: 27538435). In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. (Microbial infection) May constitute a cellular target for adenovirus E3 14.7 and Bluetongue virus protein NS3 to inhibit innate immune response.

UniProt accession

MASS(Da)

65,922

GeneID

10133

Synonyms

E3-14.7K-interacting protein;FIP-2;HIP-7;Huntingtin yeast partner L;Huntingtin-interacting protein 7;Huntingtin-interacting protein L;NEMO-related protein;Optic neuropathy-inducing protein;Optineurin;OPTN;TFIIIA-IntP;Transcription factor IIIA-interacting protein

Gene names

OPTN

Research area

Immunology; Signal Transduction

Target protein

OPTN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3579Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 1.988 1.968 1.917
1.948
10ng/ml 1.368 1.383 1.331
1.398
5ng/ml 0.705 0.7 0.649
0.696
2.5ng/ml 0.415 0.387 0.336
0.36
1.25ng/ml 0.137 0.137 0.086
0.137
0ng/ml 0.056 0.051 0
0.046

Precision

We measured random samples of E3579Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.1 0.31 5.1
Intra-Assay 2 18 9.5 0.32 3.4
Intra-Assay 3 18 3.9 0.16 4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3579Hu

    $320.00

  • 96T

    E3579Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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