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Home > Product > ELISA Kit > Human Nlr Family, Card Domain Containing 4, NLRC4 ELISA Kit

Human Nlr Family, Card Domain Containing 4, NLRC4 ELISA Kit

Human Nlr Family, Card Domain Containing 4, NLRC4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nlr Family, Card Domain Containing 4

Code

E3582Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.15ng/L

Detection range

10-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed: 15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

UniProt accession

MASS(Da)

116,159

GeneID

58484

Synonyms

CARD, LRR, and NACHT-containing protein;Caspase recruitment domain-containing protein 12;Clan protein;Ice protease-activating factor;Ipaf;NLR family CARD domain-containing protein 4;NLRC 4;NLRC4

Gene names

NLRC4

Research area

Others

Target protein

NLRC4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3582Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.675 2.667 2.613
2.658
1000ng/L 1.507 1.437 1.383
1.366
500ng/L 0.82 0.777 0.723
0.733
250ng/L 0.493 0.446 0.392
0.399
125ng/L 0.285 0.276 0.222
0.266
0ng/L 0.045 0.054 0
0.062

Precision

We measured random samples of E3582Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 828.9 47.21 5.7
Intra-Assay 2 18 1316.7 38.83 2.9
Intra-Assay 3 18 245.4 16.94 6.9

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E3582Hu

    $320.00

  • 96T

    E3582Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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