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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 10, ATG10 ELISA Kit

Mouse Autophagy Related Protein 10, ATG10 ELISA Kit

Mouse Autophagy Related Protein 10, ATG10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.69ng/L

Detection range: 6-1900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.69ng/L

Detection range: 6-1900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy related protein 10

Code

E2643Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.69ng/L

Detection range

6-1900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.

UniProt accession

MASS(Da)

24,577

GeneID

66795

Synonyms

5330424L23Rik;5430428K15Rik;Agp10;Apg;APG10;Apg10l;APG10-like;Apg10p;ATG10;Atg10l;Autophagy-related protein 10;mAPG10;Ubiquitin-like-conjugating enzyme ATG10;

Gene names

Atg10

Research area

Cell Biology; Neuroscience; Cancer; Cardiovascular; Metabolism

Target protein

ATG10

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2643Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000ng/L 2.037 2.212 2.153
2.387
500ng/L 1.52 1.537 1.478
1.555
250ng/L 1.194 1.092 1.033
0.99
125ng/L 0.667 0.647 0.588
0.627
62.5ng/L 0.439 0.45 0.39
0.46
0ng/L 0.06 0.059 0
0.059

Precision

We measured random samples of E2643Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 560.84 30.02 5.35
Intra-Assay 2 18 108.22 8.85 8.18
Intra-Assay 3 18 159.88 9.94 6.22

Protocols

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  • Size

    Code

    Price

  • 48T

    E2643Mo

    $320.00

  • 96T

    E2643Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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