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Home > Product > ELISA Kit > Mouse Autophagy Related Protein 12, ATG12 ELISA Kit

Mouse Autophagy Related Protein 12, ATG12 ELISA Kit

Mouse Autophagy Related Protein 12, ATG12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.12ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 4.12ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy related protein 12

Code

E2644Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.12ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection.

UniProt accession

MASS(Da)

15,207

GeneID

67526

Synonyms

4931423H11Rik;A330058M13Rik;Apg;Apg12l;APG12-like;ATG12;Atg12l;Autophagy-related protein 12;Ubiquitin-like protein ATG12;

Gene names

Atg12

Research area

Cell Biology; Cancer; Cardiovascular; Metabolism

Target protein

ATG12

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2644Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.28 2.356 2.29
2.433
400ng/L 1.417 1.419 1.353
1.422
200ng/L 0.858 0.835 0.769
0.811
100ng/L 0.513 0.461 0.395
0.409
50ng/L 0.316 0.322 0.256
0.328
0ng/L 0.057 0.066 0
0.075

Precision

We measured random samples of E2644Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 210.11 13.51 6.43
Intra-Assay 2 18 561.1 20.96 3.73
Intra-Assay 3 18 78.96 5.32 6.74

Protocols

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  • Size

    Code

    Price

  • 48T

    E2644Mo

    $320.00

  • 96T

    E2644Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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