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Home > Product > ELISA Kit > Human DNA Topoisomerase 1, TOP1 ELISA Kit

Human DNA Topoisomerase 1, TOP1 ELISA Kit

Human DNA Topoisomerase 1, TOP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dna Topoisomerase 1

Code

E3605Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.12ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

UniProt accession

MASS(Da)

90,726

GeneID

7150

Synonyms

DNA topoisomerase 1;DNA topoisomerase I;TOP 1;TOP1

Gene names

TOP1

Research area

Epigenetics and Nuclear Signaling

Target protein

TOP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3605Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 1.854 1.833 1.777
1.813
20ng/ml 1.05 1.094 1.038
1.138
10ng/ml 0.503 0.46 0.404
0.417
5ng/ml 0.185 0.172 0.116
0.159
2.5ng/ml 0.15 0.145 0.088
0.139
0ng/ml 0.048 0.056 0
0.064

Precision

We measured random samples of E3605Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 23.6 0.83 3.5
Intra-Assay 2 18 22.2 0.83 3.7
Intra-Assay 3 18 11.8 0.48 4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3605Hu

    $320.00

  • 96T

    E3605Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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