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Home > Product > ELISA Kit > Human Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Human Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Human Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hypoxanthine-Guanine Phosphoribosyltransferase

Code

E3638Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

UniProt accession

MASS(Da)

24,579

GeneID

3251

Synonyms

HGPRT;HGPRTase;HPRT 1;HPRT1;Hypoxanthine-guanine phosphoribosyltransferase

Gene names

HPRT1

Research area

Epigenetics and Nuclear Signaling

Target protein

HPRT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3638Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.101 2.197 2.162
2.293
6ng/ml 1.382 1.301 1.265
1.219
3ng/ml 0.721 0.723 0.688
0.725
1.5ng/ml 0.404 0.461 0.425
0.517
0.75ng/ml 0.212 0.202 0.167
0.191
0ng/ml 0.03 0.035 0
0.041

Precision

We measured random samples of E3638Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.6 0.23 3.5
Intra-Assay 2 18 3.7 0.23 6.2
Intra-Assay 3 18 2.3 0.14 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E3638Hu

    $320.00

  • 96T

    E3638Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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