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Home > Product > ELISA Kit > Human Methylcytosine Dioxygenase Tet2, TET2 ELISA Kit

Human Methylcytosine Dioxygenase Tet2, TET2 ELISA Kit

Human Methylcytosine Dioxygenase Tet2, TET2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Methylcytosine Dioxygenase Tet2

Code

E3647Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

UniProt accession

MASS(Da)

223,811

GeneID

54790

Synonyms

Methylcytosine dioxygenase TET2;TET 2;TET2

Gene names

TET2

Research area

Others

Target protein

TET2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3647Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.075 2.168 2.108
2.261
12ng/ml 1.602 1.607 1.546
1.612
6ng/ml 1.096 1.09 1.029
1.084
3ng/ml 0.631 0.649 0.588
0.666
1.5ng/ml 0.47 0.478 0.417
0.486
0ng/ml 0.065 0.061 0
0.056

Precision

We measured random samples of E3647Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.2 0.55 4.9
Intra-Assay 2 18 6.5 0.5 7.7
Intra-Assay 3 18 2 0.15 7.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3647Hu

    $320.00

  • 96T

    E3647Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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