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Home > Product > ELISA Kit > Bovine Peptidoglycan Recognition Protein 1, PGLYRP1 ELISA Kit

Bovine Peptidoglycan Recognition Protein 1, PGLYRP1 ELISA Kit

Bovine Peptidoglycan Recognition Protein 1, PGLYRP1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.091ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.091ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Peptidoglycan Recognition Protein 1

Code

E2303Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.091ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity (PubMed: 11880375, PubMed: 16394004). Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement. Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system. Promotes also the activation of lymphocytes that become able to kill virus-infected cells as well as tumor cells by modulating the spectrum of their target-cell specificity. Induction of cytotoxicity on monocyte surface requires interaction with TREM1 receptor (By similarity).

UniProt accession

MASS(Da)

21,063

GeneID

282305

Synonyms

OBP;Oligosaccharide-binding protein;Peptidoglycan recognition protein 1;Peptidoglycan recognition protein short;PGLYRP 1;PGLYRP1;PGRP-S

Gene names

PGLYRP1

Research area

Others

Target protein

PGLYRP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2303Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.487 2.411 2.294
2.336
16ng/ml 1.722 1.663 1.545
1.603
8ng/ml 1.058 1.076 0.958
1.094
4ng/ml 0.457 0.472 0.355
0.488
2ng/ml 0.342 0.335 0.218
0.328
0ng/ml 0.124 0.118 0
0.112

Precision

We measured random samples of E2303Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.23 0.31 4.24
Intra-Assay 2 18 14.85 0.47 3.18
Intra-Assay 3 18 4.01 0.19 4.79

Protocols

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  • Size

    Code

    Price

  • 96T

    E2303Bo

    $458.00

  • 48T

    E2303Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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