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Home > Product > ELISA Kit > Human Mac-2-binding Protein, M2BP ELISA Kit

Human Mac-2-binding Protein, M2BP ELISA Kit

Human Mac-2-binding Protein, M2BP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Mac-2-binding Protein

Code

E4379hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.092ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation.

UniProt accession

MASS(Da)

102,193

GeneID

27085

Synonyms

hMTBP;MAC2BP;Mdm2-binding protein;MDM2BP

Gene names

MTBP

Research area

Cancer

Target protein

MAC2BP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4379hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.201 2.142 2.108
2.082
16ng/ml 1.297 1.32 1.286
1.342
8ng/ml 0.596 0.669 0.635
0.741
4ng/ml 0.347 0.384 0.35
0.42
2ng/ml 0.185 0.173 0.14
0.161
0ng/ml 0.051 0.033 0
0.016

Precision

We measured random samples of E4379hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.57 0.35 4.66
Intra-Assay 2 18 19.4 0.57 2.93
Intra-Assay 3 18 9.31 0.4 4.27

Protocols

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  • Size

    Code

    Price

  • 48T

    E4379hu

    $320.00

  • 96T

    E4379hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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