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Home > Product > ELISA Kit > Human Ribosomal Protein S6 Kinase Beta-1, RPS6KB1 ELISA Kit

Human Ribosomal Protein S6 Kinase Beta-1, RPS6KB1 ELISA Kit

Human Ribosomal Protein S6 Kinase Beta-1, RPS6KB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ribosomal Protein S6 Kinase Beta-1

Code

E3712Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (PubMed: 11500364, PubMed: 12801526, PubMed: 14673156, PubMed: 15071500, PubMed: 15341740, PubMed: 16286006, PubMed: 17052453, PubMed: 17053147, PubMed: 17936702, PubMed: 18952604, PubMed: 19085255, PubMed: 19720745, PubMed: 19935711, PubMed: 19995915, PubMed: 23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed: 28178239).

UniProt accession

MASS(Da)

59,140

GeneID

6198

Synonyms

70 kDa ribosomal protein S6 kinase 1;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6KA;p70 S6K-alpha;p70-S6K 1;P70S6K1;Ribosomal protein S6 kinase beta-1;Ribosomal protein S6 kinase I;RPS6KB1;S6K1;S6K-beta-1;Serine/threonine-protein kinase 14A;

Gene names

RPS6KB1

Research area

Others

Target protein

RPS6KB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3712Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.841 1.959 1.913
2.078
6ng/ml 1.131 1.113 1.067
1.094
3ng/ml 0.55 0.541 0.495
0.531
1.5ng/ml 0.283 0.243 0.198
0.203
0.75ng/ml 0.114 0.112 0.066
0.11
0ng/ml 0.03 0.046 0
0.061

Precision

We measured random samples of E3712Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.3 0.1 4.3
Intra-Assay 2 18 7.5 0.2 2.7
Intra-Assay 3 18 5.2 0.26 5.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E3712Hu

    $320.00

  • 96T

    E3712Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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