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Home > Product > ELISA Kit > Human DNA Damage-inducible Transcript 4 Protein, DDIT4 ELISA Kit

Human DNA Damage-inducible Transcript 4 Protein, DDIT4 ELISA Kit

Human DNA Damage-inducible Transcript 4 Protein, DDIT4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.018ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.018ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

DNA damage-inducible transcript 4 protein

Code

E5190Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.018ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.

UniProt accession

MASS(Da)

25,371

GeneID

54541

Synonyms

DDIT4;Dig2;DNA damage-inducible transcript 4 protein;HIF-1 responsive protein RTP801;Protein regulated in development and DNA damage response 1;REDD1;REDD-1

Gene names

DDIT4

Research area

Cardiovascular

Target protein

DDIT4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5190Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.345 2.341 2.297
2.337
6ng/ml 1.842 1.839 1.795
1.836
3ng/ml 1.11 1.143 1.099
1.177
1.5ng/ml 0.666 0.708 0.664
0.75
0.75ng/ml 0.399 0.378 0.334
0.357
0ng/ml 0.059 0.044 0
0.03

Precision

We measured random samples of E5190Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.86 0.21 4.34
Intra-Assay 2 18 2.43 0.17 6.94
Intra-Assay 3 18 0.86 0.06 6.47

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5190Hu

    $320.00

  • 96T

    E5190Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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