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Home > Product > ELISA Kit > Human Telomere Repeat Binding Factor 1, TRF1 ELISA Kit

Human Telomere Repeat Binding Factor 1, TRF1 ELISA Kit

Human Telomere Repeat Binding Factor 1, TRF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Telomere Repeat Binding Factor 1

Code

E3738Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

UniProt accession

MASS(Da)

50,246

GeneID

7013

Synonyms

NIMA-interacting protein 2;Telomeric protein Pin2/TRF1;Telomeric repeat-binding factor 1;TERF 1;TERF1;TTAGGG repeat-binding factor 1

Gene names

TERF1

Research area

Others

Target protein

TERF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3738Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.668 2.66 2.62
2.652
10ng/ml 1.591 1.483 1.442
1.374
5ng/ml 0.769 0.842 0.802
0.915
2.5ng/ml 0.51 0.471 0.431
0.432
1.25ng/ml 0.279 0.255 0.215
0.232
0ng/ml 0.037 0.04 0
0.044

Precision

We measured random samples of E3738Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.8 0.24 6.2
Intra-Assay 2 18 6.9 0.42 6.1
Intra-Assay 3 18 12.2 0.4 3.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E3738Hu

    $320.00

  • 96T

    E3738Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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