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Home > Product > ELISA Kit > Human Glutathione Synthetase, GSS ELISA Kit

Human Glutathione Synthetase, GSS ELISA Kit

Human Glutathione Synthetase, GSS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028U/mL

Detection range: 0.05-20U/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028U/mL

Detection range: 0.05-20U/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glutathione synthetase

Code

E5347Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028U/mL

Detection range

0.05-20U/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glutathione synthetase (GSS) (EC 6.3.2.3) is the second enzyme in the glutathione (GSH) biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Glutathione synthetase is also a potent antioxidant. It is found in many species including bacteria, yeast, mammals, and plants. In humans, defects in GSS are inherited in an autosomal recessive way and are the cause of severe metabolic acidosis, 5-oxoprolinuria, increased rate of haemolysis, and defective function of the central nervous system. Deficiencies in GSS can cause a spectrum of deleterious symptoms in plants and human beings alike.

UniProt accession

MASS(Da)

52,385

GeneID

2937

Synonyms

Glutathione Synthase;Glutathione synthetase;GSH synthetase;GSHS;GSH-S;GSS;HEL-S-64p;HEL-S-88n

Gene names

GSS

Research area

Signal Transduction; Neuroscience; Cell Biology; Metabolism

Target protein

GSS

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5347Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8U/mL 2.32 2.206 2.163
2.093
6.4U/mL 1.259 1.327 1.284
1.394
3.2U/mL 0.815 0.828 0.784
0.84
1.6U/mL 0.49 0.448 0.405
0.406
0.8U/mL 0.23 0.21 0.167
0.19
0U/mL 0.049 0.043 0
0.038

Precision

We measured random samples of E5347Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.88 0.23 5.88
Intra-Assay 2 18 8.73 0.35 4
Intra-Assay 3 18 2.53 0.18 6.97

Protocols

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5347Hu

    $320.00

  • 96T

    E5347Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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