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Home > Product > ELISA Kit > Human High Sensitive Interleukin 4, HS IL-4 ELISA Kit

Human High Sensitive Interleukin 4, HS IL-4 ELISA Kit

Human High Sensitive Interleukin 4, HS IL-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

High Sensitive Interleukin 4

Code

E3762Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.49ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in at least several B-cell activation processes as well as of other cell types (PubMed: 3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity).

UniProt accession

MASS(Da)

17,492

GeneID

3565

Synonyms

B-cell stimulatory factor 1;Binetrakin;BSF-1;IL 4;IL4;IL-4;Interleukin-4;Lymphocyte stimulatory factor 1;Pitrakinra;

Gene names

IL4

Research area

Immunology; Cancer

Target protein

IL4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3762Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.105 2.113 2.07
2.121
400ng/L 1.025 1.048 1.006
1.071
200ng/L 0.509 0.476 0.434
0.444
100ng/L 0.214 0.232 0.189
0.249
50ng/L 0.096 0.121 0.079
0.146
0ng/L 0.046 0.043 0
0.039

Precision

We measured random samples of E3762Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 184.9 9.2 5
Intra-Assay 2 18 348.7 17.1 4.9
Intra-Assay 3 18 633.4 15.64 2.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E3762Hu

    $320.00

  • 96T

    E3762Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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