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Home > Product > ELISA Kit > Human Rho-associated Protein Kinase 1, ROCK1 ELISA Kit

Human Rho-associated Protein Kinase 1, ROCK1 ELISA Kit

Human Rho-associated Protein Kinase 1, ROCK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rho-Associated Protein Kinase 1

Code

E3773Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579, PubMed: 23093407, PubMed: 23355470). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed: 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed: 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed: 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed: 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed: 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).

UniProt accession

MASS(Da)

158,175

GeneID

6093

Synonyms

p160 ROCK-1;p160ROCK;Renal carcinoma antigen NY-REN-35;Rho-associated protein kinase 1;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK 1;ROCK1;ROCK-I;

Gene names

ROCK1

Research area

Others

Target protein

ROCK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3773Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.962 1.85 1.814
1.738
6ng/ml 0.986 1.033 0.997
1.079
3ng/ml 0.476 0.53 0.493
0.583
1.5ng/ml 0.159 0.211 0.175
0.263
0.75ng/ml 0.054 0.091 0.055
0.129
0ng/ml 0.031 0.036 0
0.041

Precision

We measured random samples of E3773Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.6 0.25 4.5
Intra-Assay 2 18 7.7 0.19 2.5
Intra-Assay 3 18 2.1 0.09 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3773Hu

    $320.00

  • 96T

    E3773Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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