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Home > Product > ELISA Kit > Human Glycocalicin, GC ELISA Kit

Human Glycocalicin, GC ELISA Kit

Human Glycocalicin, GC ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028μg/mL

Detection range: 0.05-20ug/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028μg/mL

Detection range: 0.05-20ug/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glycocalicin

Code

E3792Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028μg/mL

Detection range

0.05-20ug/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glycoprotein that regulates critical steps during fertilization and also has immunomonomodulatory effects. Four glycoforms, namely glycodelin-S, -A, -F and -C have been identified in reproductive tissues that differ in glycosylation and biological activity. Glycodelin-A has contraceptive and immunosuppressive activities (PubMed: 9918684, PubMed: 7531163). Glycodelin-C stimulates binding of spermatozoa to the zona pellucida (PubMed: 17192260). Glycodelin-F inhibits spermatozoa-zona pellucida binding and significantly suppresses progesterone-induced acrosome reaction of spermatozoa (PubMed: 12672671). Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa (PubMed: 15883155).

UniProt accession

MASS(Da)

20,624

GeneID

5047

Synonyms

GD;Glycodelin;PAEG;PAEP;PEG;Placental protein 14;PP14;Pregnancy-associated endometrial alpha-2 globulin;Progestagen-associated endometrial protein;Progesterone-associated endometrial protein;ZIF-1;Zona-binding inhibitory factor-1

Gene names

PAEP

Research area

Others

Target protein

PAEP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3792Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12μg/mL 2.241 2.178 2.128
2.116
6μg/mL 1.567 1.537 1.486
1.507
3μg/mL 0.969 1.041 0.991
1.112
1.5μg/mL 0.753 0.7 0.65
0.647
0.75μg/mL 0.412 0.423 0.372
0.433
0μg/mL 0.064 0.05 0
0.036

Precision

We measured random samples of E3792Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.2 0.33 4.7
Intra-Assay 2 18 1.6 0.12 7.5
Intra-Assay 3 18 2.4 0.18 7.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E3792Hu

    $320.00

  • 96T

    E3792Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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