Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Rac-alpha Serine, Threonine-protein Kinase, AKT1 ELISA Kit

Human Rac-alpha Serine, Threonine-protein Kinase, AKT1 ELISA Kit

Human Rac-alpha Serine, Threonine-protein Kinase, AKT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rac-Alpha Serine/Threonine-Protein Kinase

Code

E3841Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis (PubMed: 15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates (PubMed: 15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported (PubMed: 15526160, PubMed: 11882383, PubMed: 21620960, PubMed: 21432781). AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface (By similarity). Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling (By similarity). Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport (PubMed: 11994271). AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity (By similarity). Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven (By similarity). AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase) (PubMed: 11154276). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis (PubMed: 11154276). AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1 (PubMed: 12150915). AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization (PubMed: 10358075). In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319' (PubMed: 10358075). FOXO3 and FOXO4 are phosphorylated on equivalent sites (PubMed: 10358075). AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein) (PubMed: 9829964). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1 (PubMed: 9829964). AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis (By similarity). Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis (By similarity). Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity (By similarity). The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and Insulin-like growth factor I (IGF-I) (PubMed: 12176338, PubMed: 12964941). AKT mediates the antiapoptotic effects of IGF-I (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed: 19934221). May be involved in the regulation of the placental development (By similarity). Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3 (PubMed: 17726016). Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation (PubMed: 20086174, PubMed: 20231902). Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation (PubMed: 19592491). Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity (PubMed: 10576742). Phosphorylation of BAD stimulates its pro-apoptotic activity (PubMed: 10926925). Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53 (PubMed: 23431171). Phosphorylates palladin (PALLD), modulating cytoskeletal organization and cell motility (PubMed: 20471940). Phosphorylates prohibitin (PHB), playing an important role in cell metabolism and proliferation (PubMed: 18507042). Phosphorylates CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization (PubMed: 16982699). These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation (PubMed: 16139227). Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation (PubMed: 20682768). Phosphorylates PCK1 at 'Ser-90', reducing the binding affinity of PCK1 to oxaloacetate and changing PCK1 into an atypical protein kinase activity using GTP as donor (PubMed: 32322062).

UniProt accession

MASS(Da)

55,686

GeneID

207

Synonyms

AKT 1;AKT1;PKB;PKB alpha;Protein kinase B;Protein kinase B alpha;Proto-oncogene c-Akt;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha;

Gene names

AKT1

Research area

Signal Transduction

Target protein

AKT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3841Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8ng/ml 2.099 1.978 1.93
1.856
6.4ng/ml 0.964 0.962 0.914
0.96
3.2ng/ml 0.532 0.526 0.478
0.52
1.6ng/ml 0.222 0.243 0.195
0.264
0.8ng/ml 0.081 0.122 0.074
0.164
0ng/ml 0.044 0.048 0
0.052

Precision

We measured random samples of E3841Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3 0.18 6
Intra-Assay 2 18 7.1 0.29 4.1
Intra-Assay 3 18 8.2 0.19 2.3

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E3841Hu

    $320.00

  • 96T

    E3841Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up