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Home > Product > ELISA Kit > Human Protein Phosphatase 1B, PPM1B ELISA Kit

Human Protein Phosphatase 1B, PPM1B ELISA Kit

Human Protein Phosphatase 1B, PPM1B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Phosphatase 1B

Code

E3842Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

UniProt accession

MASS(Da)

52,643

GeneID

5495

Synonyms

PP2C-beta;PPM 1B;PPM1B;Protein phosphatase 1B;Protein phosphatase 2C isoform beta

Gene names

PPM1B

Research area

Cell Biology

Target protein

PPM1B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3842Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.243 2.254 2.21
2.266
4ng/ml 1.479 1.472 1.427
1.464
2ng/ml 0.903 0.911 0.867
0.92
1ng/ml 0.424 0.46 0.415
0.496
0.5ng/ml 0.247 0.248 0.204
0.249
0ng/ml 0.054 0.045 0
0.035

Precision

We measured random samples of E3842Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.2 0.18 4.3
Intra-Assay 2 18 2 0.09 4.3
Intra-Assay 3 18 1 0.05 4.8

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3842Hu

    $320.00

  • 96T

    E3842Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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