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Home > Product > ELISA Kit > Human 25-Hydroxycholesterol 7-alpha-hydroxylase, CYP7B1 ELISA Kit

Human 25-Hydroxycholesterol 7-alpha-hydroxylase, CYP7B1 ELISA Kit

Human 25-Hydroxycholesterol 7-alpha-hydroxylase, CYP7B1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

25-Hydroxycholesterol 7-Alpha-Hydroxylase

Code

E3861Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis (PubMed: 25201972, PubMed: 10748047). Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver (PubMed: 25201972, PubMed: 10748047). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed: 25201972, PubMed: 10748047).

UniProt accession

MASS(Da)

54,116

GeneID

51302

Synonyms

24-hydroxycholesterol 7-alpha-hydroxylase;CYP39A1;Cytochrome P450 39A1;hCYP39A1;Oxysterol 7-alpha-hydroxylase

Gene names

CYP39A1

Research area

Others

Target protein

CYP39A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3861Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected

Precision

We measured random samples of E3861Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.45 0.09 6.02
Intra-Assay 2 18 3.32 0.1 3.1
Intra-Assay 3 18 2.65 0.12 4.57

Protocols

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  • Size

    Code

    Price

  • 48T

    E3861Hu

    $320.00

  • 96T

    E3861Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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