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Home > Product > ELISA Kit > Human Sparc-like Protein 1, SPARCL1 ELISA Kit

Human Sparc-like Protein 1, SPARCL1 ELISA Kit

Human Sparc-like Protein 1, SPARCL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.049ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.049ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

SPARC-like protein 1

Code

E6207Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.049ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

SPARC-like protein 1 (SPARCL1 or SC1), also known as hevin (short for high endothelial venule protein), is a secreted protein with high structural similarity to SPARC. It interacts with the extracellular matrix to create intermediate states of cell adhesion. Due to its dynamic extracellular roles, being implicated in cancer metastasis and inflammation, it is considered a matricellular protein. In humans hevin is encoded by the SPARCL1 gene.

UniProt accession

MASS(Da)

75,208

GeneID

8404

Synonyms

Ecm2;Hevin;High endothelial venule protein;MAST 9;MAST9;PIG33;SC1;SPARCL1;SPARC-like protein 1;

Gene names

SPARCL1

Research area

Cancer

Target protein

SPARCL1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6207Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.24 2.303 2.238
2.366
12ng/mL 1.276 1.365 1.299
1.453
6ng/mL 0.844 0.801 0.736
0.758
3ng/mL 0.519 0.505 0.44
0.491
1.5ng/mL 0.28 0.301 0.236
0.322
0ng/mL 0.072 0.065 0
0.059

Precision

We measured random samples of E6207Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.37 0.28 8.45
Intra-Assay 2 18 11.9 0.4 3.33
Intra-Assay 3 18 6.58 0.42 6.37

Protocols

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  • Size

    Code

    Price

  • 48T

    E6207Hu

    $320.00

  • 96T

    E6207Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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