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Home > Product > ELISA Kit > Human Secreted Frizzled Related Protein 3, SFRP3 ELISA Kit

Human Secreted Frizzled Related Protein 3, SFRP3 ELISA Kit

Human Secreted Frizzled Related Protein 3, SFRP3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Secreted Frizzled Related Protein 3

Code

E3897Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

UniProt accession

MASS(Da)

36,254

GeneID

2487

Synonyms

Frezzled;Fritz;Frizzled-related protein 1;FRZB;FrzB-1;Secreted frizzled-related protein 3;sFRP-3

Gene names

FRZB

Research area

Signal Transduction

Target protein

FRZB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3897Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.227 2.202 2.154
2.177
10ng/ml 1.125 1.162 1.114
1.199
5ng/ml 0.635 0.654 0.606
0.673
2.5ng/ml 0.493 0.494 0.446
0.495
1.25ng/ml 0.232 0.242 0.194
0.252
0ng/ml 0.056 0.048 0
0.041

Precision

We measured random samples of E3897Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.4 0.36 6.7
Intra-Assay 2 18 7.4 0.47 6.3
Intra-Assay 3 18 13.2 0.39 3

Protocols

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  • Size

    Code

    Price

  • 48T

    E3897Hu

    $320.00

  • 96T

    E3897Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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