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Home > Product > ELISA Kit > Human Cancer/Testis Antigen 1, CTAG1A ELISA Kit

Human Cancer/Testis Antigen 1, CTAG1A ELISA Kit

Human Cancer/Testis Antigen 1, CTAG1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.012ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.012ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cancer/testis antigen 1

Code

E6592Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.012ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cancer/testis antigen 1 also known as LAGE2 or LAGE2B is a protein that in humans is encoded by the CTAG1B gene. It is most often referenced by its alias NY-ESO-1. Cancer/Testis Antigen 1B is a protein belonging to the family of Cancer Testis Antigens (CTA) that are expressed in a variety of malignant tumours at the mRNA and protein levels, but also restricted to testicular germ cells in normal adult tissues. A clone of CTAG gene was originally identified by immunological methods in oesophageal carcinoma using patient serum.[6] The aberrant re-expression of CTAs is induced by molecular mechanisms including DNA demethylation, histone post-translational modification, and microRNA-mediated regulation. The effect of DNA demethylation is evident by the capability of demethylating agents, such as 5-aza-2-deoxycytidine, to induce CTAs re-expression in tumour cells but not in normal epithelial cells.

UniProt accession

MASS(Da)

17,992

GeneID

1485;246100

Synonyms

Autoimmunogenic cancer/testis antigen NY-ESO-1;Cancer/testis antigen 1;Cancer/testis antigen 6.1;CT6.1;CTAG;CTAG1A;ESO1;L antigen family member 2;LAGE-2;LAGE2A;LAGE2B;NY-ESO-1

Gene names

CTAG1A; CTAG1B

Research area

Tags & Cell Markers; Cancer

Target protein

CTAG1A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6592Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.271 2.251 2.209
2.23
6ng/ml 1.375 1.32 1.279
1.266
3ng/ml 0.67 0.665 0.623
0.659
1.5ng/ml 0.442 0.432 0.391
0.423
0.75ng/ml 0.251 0.225 0.184
0.199
0ng/ml 0.04 0.041 0
0.043

Precision

We measured random samples of E6592Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.38 0.16 4.73
Intra-Assay 2 18 6.48 0.16 2.46
Intra-Assay 3 18 1.79 0.11 6.15

Protocols

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  • Size

    Code

    Price

  • 48T

    E6592Hu

    $320.00

  • 96T

    E6592Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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