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Home > Product > ELISA Kit > Human Bdnf/Nt-3 Growth Factors Receptor, NTRK2 ELISA Kit

Human Bdnf/Nt-3 Growth Factors Receptor, NTRK2 ELISA Kit

Human Bdnf/Nt-3 Growth Factors Receptor, NTRK2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

BDNF/NT-3 growth factors receptor

Code

E6633Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.13ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed:7574684, PubMed:15494731). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

UniProt accession

MASS(Da)

91,999

GeneID

4915

Synonyms

BDNF/NT-3 growth factors receptor;DEE58;EIEE58;GP145-TrkB;Neurotrophic tyrosine kinase receptor type 2;NTRK2;OBHD;TRKB;trk-B;TrkB tyrosine kinase;Tropomyosin-related kinase B

Gene names

NTRK2

Research area

Neuroscience; Cancer; Metabolism

Target protein

NTRK2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6633Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.1 2.119 2.07
2.138
24ng/ml 1.494 1.61 1.562
1.727
12ng/ml 1.093 1.084 1.036
1.075
6ng/ml 0.757 0.712 0.664
0.668
3ng/ml 0.486 0.473 0.424
0.46
0ng/ml 0.054 0.049 0
0.044

Precision

We measured random samples of E6633Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.79 0.25 8.96
Intra-Assay 2 18 22.58 1.34 5.94
Intra-Assay 3 18 13.79 1.09 7.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E6633Hu

    $320.00

  • 96T

    E6633Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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