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Home > Product > ELISA Kit > Human 5-oxoprolinase, OPLAH ELISA Kit

Human 5-oxoprolinase, OPLAH ELISA Kit

Human 5-oxoprolinase, OPLAH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.016ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.016ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

5-oxoprolinase

Code

E6640Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.016ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

UniProt accession

MASS(Da)

137,457

GeneID

26873

Synonyms

5-Opase;5-oxo-L-prolinase;5-oxoprolinase;OPLA;OPLAH;OPLAHD;Pyroglutamase

Gene names

OPLAH

Research area

Neuroscience; Cardiovascular; Metabolism

Target protein

OPLAH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6640Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.426 2.43 2.394
2.434
4ng/ml 1.323 1.349 1.314
1.375
2ng/ml 0.706 0.661 0.625
0.615
1ng/ml 0.419 0.355 0.32
0.291
0.5ng/ml 0.179 0.159 0.123
0.138
0ng/ml 0.049 0.035 0
0.021

Precision

We measured random samples of E6640Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.16 0.12 2.98
Intra-Assay 2 18 2.19 0.1 4.65
Intra-Assay 3 18 2.1 0.12 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E6640Hu

    $320.00

  • 96T

    E6640Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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