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Home > Product > ELISA Kit > Human DNA Topoisomerase 2-alpha, TOP2A ELISA Kit

Human DNA Topoisomerase 2-alpha, TOP2A ELISA Kit

Human DNA Topoisomerase 2-alpha, TOP2A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.78ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.78ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

DNA topoisomerase 2-alpha

Code

E6652Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.78ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

UniProt accession

MASS(Da)

174,385

GeneID

7153

Synonyms

DNA topoisomerase 2-alpha;DNA topoisomerase II, alpha isozyme;TOP2;TOP2A;TOP2alpha;TOPIIA;TP2A

Gene names

TOP2A

Research area

Epigenetics and Nuclear Signaling; Cancer

Target protein

TOP2A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6652Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 1.956 2.072 2.027
2.187
1000ng/L 1.41 1.445 1.4
1.48
500ng/L 0.69 0.646 0.601
0.602
250ng/L 0.354 0.354 0.308
0.353
125ng/L 0.192 0.195 0.15
0.199
0ng/L 0.037 0.045 0
0.053

Precision

We measured random samples of E6652Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1425.97 72.55 5.09
Intra-Assay 2 18 378.49 14.35 3.79
Intra-Assay 3 18 657.54 30.84 4.69

Protocols

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  • Size

    Code

    Price

  • 48T

    E6652Hu

    $320.00

  • 96T

    E6652Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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