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Home > Product > ELISA Kit > Human Aurora Kinase A, AURKA ELISA Kit

Human Aurora Kinase A, AURKA ELISA Kit

Human Aurora Kinase A, AURKA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aurora Kinase A

Code

E6653Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Aurora kinase A also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. Aurora A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora A is activated by one or more phosphorylations[7] and its activity peaks during the G2 phase to M phase transition in the cell cycle.

UniProt accession

MASS(Da)

45,809

GeneID

6790

Synonyms

AIK;ARK1;ARK-1;AURA;AURKA;Aurora 2;Aurora kinase A;Aurora/IPL1-related kinase 1;Aurora-related kinase 1;Breast tumor-amplified kinase;BTAK;hARK1;PPP1R47;Serine/threonine-protein kinase 15;Serine/threonine-protein kinase 6;Serine/threonine-protein kinase aurora-A;STK6;STK7

Gene names

AURKA

Research area

Cell Biology; Signal Transduction; Cancer

Target protein

AURKA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6653Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.416 2.355 2.293
2.294
6ng/ml 1.243 1.266 1.204
1.289
3ng/ml 0.794 0.841 0.778
0.888
1.5ng/ml 0.437 0.421 0.358
0.404
0.75ng/ml 0.273 0.288 0.226
0.303
0ng/ml 0.06 0.062 0
0.065

Precision

We measured random samples of E6653Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.31 0.25 2.96
Intra-Assay 2 18 3.89 0.21 5.33
Intra-Assay 3 18 2.43 0.2 8.13

Protocols

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  • Size

    Code

    Price

  • 48T

    E6653Hu

    $320.00

  • 96T

    E6653Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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