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Home > Product > ELISA Kit > Human Aldo-Keto Reductase Family 1 Member C4, AKR1C4 ELISA Kit

Human Aldo-Keto Reductase Family 1 Member C4, AKR1C4 ELISA Kit

Human Aldo-Keto Reductase Family 1 Member C4, AKR1C4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.63ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.63ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aldo-keto reductase family 1 member C4

Code

E6721Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.63ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain (PubMed:14672942, PubMed:10998348, PubMed:7650035, PubMed:1530633, PubMed:11158055, PubMed:10634139, PubMed:19218247). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:14672942). Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity (PubMed:14672942). Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:11158055, PubMed:10998348, PubMed:14672942). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency (PubMed:14672942). Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids (PubMed:10998348, PubMed:14672942, PubMed:19218247). Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route (PubMed:2427522).

UniProt accession

MASS(Da)

37,067

GeneID

1109

Synonyms

3-alpha-HSD1;3alpha-hydroxysteroid 3-dehydrogenase;3-alpha-hydroxysteroid dehydrogenase type I;AKR1C4;Aldo-keto reductase family 1 member C4;C11;CDR;CHDR;Chlordecone reductase;DD4;DD-4;Dihydrodiol dehydrogenase 4;HAKRA;

Gene names

AKR1C4

Research area

Signal Transduction; Cancer; Metabolism

Target protein

AKR1C4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6721Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.06 1.996 1.954
1.933
1000ng/L 0.932 1.022 0.98
1.113
500ng/L 0.446 0.435 0.392
0.424
250ng/L 0.272 0.265 0.223
0.259
125ng/L 0.141 0.139 0.096
0.136
0ng/L 0.051 0.042 0
0.034

Precision

We measured random samples of E6721Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1747.15 48.64 2.78
Intra-Assay 2 18 625.43 29.09 4.65
Intra-Assay 3 18 873.08 34.29 3.93

Protocols

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  • Size

    Code

    Price

  • 48T

    E6721Hu

    $320.00

  • 96T

    E6721Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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