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Home > Product > ELISA Kit > Human Nadh-ubiquinone Oxidoreductase 75 kDa Subunit, NDUFS1 ELISA Kit

Human Nadh-ubiquinone Oxidoreductase 75 kDa Subunit, NDUFS1 ELISA Kit

Human Nadh-ubiquinone Oxidoreductase 75 kDa Subunit, NDUFS1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 11.11ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 11.11ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

NADH-ubiquinone oxidoreductase 75 kDa subunit

Code

E6754Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

11.11ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1) is an enzyme that in humans is encoded by the NDUFS1 gene. The encoded protein, NDUFS1, is the largest subunit of complex I, located on the inner mitochondrial membrane, and is important for mitochondrial oxidative phosphorylation. Mutations in this gene are associated with complex I deficiency. The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

UniProt accession

MASS(Da)

79,468

GeneID

4719

Synonyms

CI-75k;CI-75Kd;Complex I-75kD;MC1DN5;NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial;NDUFS1;PRO1304

Gene names

NDUFS1

Research area

Tags & Cell Markers; Neuroscience; Cancer; Metabolism

Target protein

NDUFS1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6754Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.044 2.17 2.127
2.296
1000ng/L 1.404 1.418 1.375
1.431
500ng/L 0.844 0.866 0.823
0.889
250ng/L 0.468 0.491 0.448
0.514
125ng/L 0.237 0.256 0.213
0.276
0ng/L 0.047 0.043 0
0.039

Precision

We measured random samples of E6754Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 415 27.42 6.61
Intra-Assay 2 18 1036.35 37.35 3.6
Intra-Assay 3 18 753.89 39.93 5.3

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E6754Hu

    $320.00

  • 96T

    E6754Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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