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Home > Product > ELISA Kit > Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 8, NDUFS8 ELISA Kit

Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 8, NDUFS8 ELISA Kit

Human Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 8, NDUFS8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.39ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.39ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8

Code

E6756Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.39ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial also known as NADH-ubiquinone oxidoreductase 23 kDa subunit, Complex I-23kD (CI-23kD), or TYKY subunit is an enzyme that in humans is encoded by the NDUFS8 gene. This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process.

UniProt accession

MASS(Da)

23,705

GeneID

4728

Synonyms

CI-23k;CI23KD;CI-23kD;Complex I-23kD;MC1DN2;NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial;NADH-ubiquinone oxidoreductase 23 kDa subunit;NDUFS8;TYKY;TYKY subunit

Gene names

NDUFS8

Research area

Tags & Cell Markers; Signal Transduction; Cancer; Metabolism

Target protein

NDUFS8

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6756Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.338 2.217 2.167
2.097
400ng/L 1.428 1.34 1.29
1.252
200ng/L 0.6 0.615 0.564
0.629
100ng/L 0.44 0.384 0.334
0.328
50ng/L 0.13 0.124 0.074
0.119
0ng/L 0.053 0.05 0
0.048

Precision

We measured random samples of E6756Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 515.14 16.84 3.27
Intra-Assay 2 18 316.85 13.96 4.41
Intra-Assay 3 18 166.5 9.19 5.52

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6756Hu

    $320.00

  • 96T

    E6756Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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