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Home > Product > ELISA Kit > Human Sequestosome-1, SQSTM1 ELISA Kit

Human Sequestosome-1, SQSTM1 ELISA Kit

Human Sequestosome-1, SQSTM1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sequestosome-1

Code

E6779Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family (PubMed:16286508, PubMed:20168092, PubMed:24128730, PubMed:28404643, PubMed:22622177). Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:24128730, PubMed:20168092). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed:29496741). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed:20452972, PubMed:28380357).

UniProt accession

MASS(Da)

47,687

GeneID

8878

Synonyms

A170;DMRV;EBI3-associated protein of 60 kDa;EBIAP;FTDALS3;NADGP;OSIL;p60;p62;p62B;PDB3;Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Sequestosome-1;SQSTM1;Ubiquitin-binding protein p62

Gene names

SQSTM1

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Cardiovascular; Metabolism; Cancer

Target protein

SQSTM1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6779Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.312 2.21 2.168
2.109
8ng/ml 1.707 1.731 1.688
1.754
4ng/ml 1.238 1.192 1.15
1.147
2ng/ml 0.599 0.647 0.604
0.695
1ng/ml 0.371 0.368 0.325
0.365
0ng/ml 0.033 0.043 0
0.052

Precision

We measured random samples of E6779Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.55 0.14 5.44
Intra-Assay 2 18 5.17 0.21 4.08
Intra-Assay 3 18 1.09 0.06 5.36

Protocols

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  • Size

    Code

    Price

  • 48T

    E6779Hu

    $320.00

  • 96T

    E6779Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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