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Home > Product > ELISA Kit > Human Neuronal Pas Domain-containing Protein 4, NPAS4 ELISA Kit

Human Neuronal Pas Domain-containing Protein 4, NPAS4 ELISA Kit

Human Neuronal Pas Domain-containing Protein 4, NPAS4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neuronal PAS domain-containing protein 4

Code

E6793Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hyppocampus (By similarity). Plays a key role in the structural and functional plasticity of neurons (By similarity). Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (By similarity). In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (By similarity). In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (By similarity). The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (By similarity). Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (By similarity). Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (PubMed:14701734). Can activate the CME (CNS midline enhancer) element (PubMed:14701734).

UniProt accession

MASS(Da)

87,117

GeneID

266743

Synonyms

bHLHe79;Class E basic helix-loop-helix protein 79;HLH-PAS transcription factor NXF;Le-PAS;Neuronal PAS domain-containing protein 4;Neuronal PAS4;NPAS4;NXF;PAS domain-containing protein 10;PASD10

Gene names

NPAS4

Research area

Neuroscience; Signal Transduction; Epigenetics and Nuclear Signaling; Metabolism

Target protein

NPAS4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6793Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 1.986 1.967 1.921
1.948
8ng/ml 0.921 0.948 0.902
0.974
4ng/ml 0.603 0.602 0.556
0.601
2ng/ml 0.151 0.147 0.101
0.144
1ng/ml 0.065 0.088 0.042
0.112
0ng/ml 0.049 0.046 0
0.043

Precision

We measured random samples of E6793Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.21 0.2 4.83
Intra-Assay 2 18 11.74 0.3 2.52
Intra-Assay 3 18 6.25 0.26 4.23

Protocols

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  • Size

    Code

    Price

  • 48T

    E6793Hu

    $320.00

  • 96T

    E6793Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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