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Home > Product > ELISA Kit > Human Scaffold Attachment Factor B1, SAFB ELISA Kit

Human Scaffold Attachment Factor B1, SAFB ELISA Kit

Human Scaffold Attachment Factor B1, SAFB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.56ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Scaffold Attachment Factor B1

Code

E3949Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.56ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed: 9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed: 12660241). Thereby acts as a negative regulator of cell proliferation (PubMed: 12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).

UniProt accession

MASS(Da)

102,642

GeneID

6294

Synonyms

HSP27 ERE-TATA-binding protein;HSP27 estrogen response element-TATA box-binding protein;SAFB;SAF-B;SAF-B1;Scaffold attachment factor B1

Gene names

SAFB

Research area

Epigenetics and Nuclear Signaling

Target protein

SAFB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3949Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.186 2.201 2.142
2.215
320ng/L 1.541 1.546 1.488
1.551
160ng/L 1.007 0.997 0.938
0.986
80ng/L 0.635 0.634 0.576
0.634
40ng/L 0.465 0.458 0.4
0.451
0ng/L 0.059 0.058 0
0.057

Precision

We measured random samples of E3949Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 400.4 22.52 5.6
Intra-Assay 2 18 63 4.38 7
Intra-Assay 3 18 141.3 8.47 6

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3949Hu

    $320.00

  • 96T

    E3949Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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