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Home > Product > ELISA Kit > Human Prolyl Endopeptidase, PREP ELISA Kit

Human Prolyl Endopeptidase, PREP ELISA Kit

Human Prolyl Endopeptidase, PREP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prolyl Endopeptidase

Code

E3950Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/mL

Detection range

0.05-30ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cleaves Peptide bonds on the C-terminal side of prolyl residues within Peptides that are up to approximately 30 amino acids long.

UniProt accession

MASS(Da)

80,700

GeneID

5550

Synonyms

PE;Post-proline cleaving enzyme;PREP;Prolyl endopeptidase

Gene names

PREP

Research area

Others

Target protein

PREP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3950Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/mL 2.269 2.286 2.244
2.303
10ng/mL 1.378 1.377 1.335
1.376
5ng/mL 0.666 0.647 0.605
0.628
2.5ng/mL 0.529 0.506 0.464
0.483
1.25ng/mL 0.219 0.228 0.186
0.236
0ng/mL 0.055 0.042 0
0.029

Precision

We measured random samples of E3950Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.1 0.24 4.6
Intra-Assay 2 18 12.1 0.45 3.7
Intra-Assay 3 18 4 0.24 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E3950Hu

    $320.00

  • 96T

    E3950Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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