Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Transcriptional Coactivator Yap1, YAP1 ELISA Kit

Human Transcriptional Coactivator Yap1, YAP1 ELISA Kit

Human Transcriptional Coactivator Yap1, YAP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Transcriptional coactivator YAP1

Code

E6874Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.093ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

UniProt accession

MASS(Da)

54,462

GeneID

10413

Synonyms

COB1;Protein yorkie homolog;Transcriptional coactivator YAP1;YAP;YAP1;YAP2;YAP65;Yes-associated protein 1;Yes-associated protein YAP65 homolog;YKI

Gene names

YAP1

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Target protein

YAP1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6874Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.319 2.23 2.195
2.141
16ng/ml 1.703 1.781 1.747
1.86
8ng/ml 1.252 1.285 1.25
1.318
4ng/ml 0.706 0.671 0.636
0.636
2ng/ml 0.384 0.368 0.333
0.351
0ng/ml 0.032 0.035 0
0.037

Precision

We measured random samples of E6874Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.69 0.2 4.21
Intra-Assay 2 18 13.92 0.95 6.83
Intra-Assay 3 18 2.23 0.09 4.23

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E6874Hu

    $320.00

  • 96T

    E6874Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up