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Home > Product > ELISA Kit > Human Phenylalanine-4-hydroxylase, PAH ELISA Kit

Human Phenylalanine-4-hydroxylase, PAH ELISA Kit

Human Phenylalanine-4-hydroxylase, PAH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.86ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.86ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Phenylalanine-4-hydroxylase

Code

E6875Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.86ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Phenylalanine hydroxylase (PAH) (EC 1.14.16.1) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. PAH is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. During the reaction, molecular oxygen is heterolytically cleaved with sequential incorporation of one oxygen atom into BH4 and phenylalanine substrate. In humans, mutations in its encoding gene, PAH, can lead to the metabolic disorder phenylketonuria.

UniProt accession

MASS(Da)

51,862

GeneID

5053

Synonyms

PAH;PH;Phe-4-monooxygenase;Phenylalanine-4-hydroxylase;PKU;PKU1

Gene names

PAH

Research area

Signal Transduction; Metabolism

Target protein

PAH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6875Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.193 2.123 2.066
2.054
600ng/L 1.337 1.341 1.284
1.345
300ng/L 0.727 0.82 0.763
0.914
150ng/L 0.404 0.4 0.343
0.396
75ng/L 0.313 0.275 0.218
0.237
0ng/L 0.058 0.057 0
0.057

Precision

We measured random samples of E6875Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 184.43 9.66 5.24
Intra-Assay 2 18 468.63 25.06 5.35
Intra-Assay 3 18 568.16 16.36 2.88

Protocols

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  • Size

    Code

    Price

  • 48T

    E6875Hu

    $320.00

  • 96T

    E6875Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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