Human Platelet Activating Factor Acetylhydrolase, PAF-AH ELISA Kit

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Home > Product > ELISA Kit > Human Platelet Activating Factor Acetylhydrolase, PAF-AH ELISA Kit

Human Platelet Activating Factor Acetylhydrolase, PAF-AH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.21ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.21ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Platelet Activating Factor Acetylhydrolase

Code

E3993Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.21ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response (PubMed: 7700381, PubMed: 8624782, PubMed: 2040620, PubMed: 16371369, PubMed: 17090529, PubMed: 10066756). At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed: 2040620, PubMed: 10504265). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position (PubMed: 2040620). Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups (PubMed: 2040620, PubMed: 8624782). Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells (PubMed: 10504265, PubMed: 10066756, PubMed: 7592717, PubMed: 11590221, PubMed: 7700381, PubMed: 18434304, PubMed: 16371369, PubMed: 8675689, PubMed: 8624782). Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled proinflammatory effects (PubMed: 2040620, PubMed: 7700381). As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall (PubMed: 17090529). Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage (PubMed: 16371369).

UniProt accession

Q13093

MASS（Da）

50,077

GeneID

7941

Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase;2-acetyl-1-alkylglycerophosphocholine esterase;Group-VIIA phospholipase A2;gVIIA-PLA2;LDL-associated phospholipase A2;LDL-PLA(2;PAF 2-acylhydrolase;PAF acetylhydrolase;PLA 2G7;PLA2G7;Platelet-activating factor acetylhydrolase

Gene names

PLA2G7

Research area

Metabolism

Target protein

PLA2G7

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3993Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.207	2.084	2.033
	1.961
1600ng/L	1.313	1.417	1.366
	1.521
800ng/L	0.562	0.615	0.564
	0.668
400ng/L	0.365	0.404	0.353
	0.444
200ng/L	0.142	0.158	0.107
	0.174
0ng/L	0.058	0.051	0
	0.043

Precision

We measured random samples of E3993Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1360.6	61.56	4.5
Intra-Assay	2	18	1496.1	54.28	3.6
Intra-Assay	3	18	445.5	17.69	4

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> Protocol Booklet

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Datasheet

Size

Code

Price

48T

E3993Hu

$320.00
96T

E3993Hu

$458.00

Add to Cart

Lead time: Within two weeks

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