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Home > Product > ELISA Kit > Canine Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Canine Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Canine Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.24ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 1.24ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Vascular Cell Adhesion Molecule 1

Code

E0020Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.24ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation (By similarity).

UniProt accession

MASS(Da)

81,412

GeneID

403982

Synonyms

CD antigen CD106;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1

Gene names

VCAM1

Research area

Cancer

Target protein

VCAM1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0020Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/ml 2.387 2.315 2.295
2.243
160ng/ml 1.188 1.189 1.17
1.191
80ng/ml 0.678 0.644 0.624
0.609
40ng/ml 0.363 0.354 0.334
0.345
20ng/ml 0.164 0.167 0.147
0.17
0ng/ml 0.019 0.02 0
0.021

Precision

We measured random samples of E0020Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 150.4 7.65 5.1
Intra-Assay 2 18 227.9 5.42 2.4
Intra-Assay 3 18 61.8 2.93 4.7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0020Ca

    $475.00

  • 48T

    E0020Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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