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Home > Product > ELISA Kit > Human Serine, Threonine-protein Kinase Ulk1, ULK1 ELISA Kit

Human Serine, Threonine-protein Kinase Ulk1, ULK1 ELISA Kit

Human Serine, Threonine-protein Kinase Ulk1, ULK1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/Threonine-Protein Kinase Ulk1

Code

E4033hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed: 18936157, PubMed: 21460634, PubMed: 21795849, PubMed: 25040165). Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed: 18936157, PubMed: 21460634, PubMed: 21795849, PubMed: 25040165). Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed: 21795849). Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed: 21460634). May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed: 18936157). Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed: 11146101). May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed: 25040165). Phosphorylates FLCN, promoting autophagy (PubMed: 25126726).

UniProt accession

MASS(Da)

112,631

GeneID

8408

Synonyms

ATG1;Autophagy-related protein 1 homolog;hATG1;Serine/threonine-protein kinase ULK1;ULK 1;ULK1;Unc-51-like kinase 1

Gene names

ULK1

Research area

Others

Target protein

ULK1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4033hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.25 2.299 2.227
2.348
4ng/ml 1.464 1.37 1.298
1.277
2ng/ml 0.864 0.831 0.759
0.797
1ng/ml 0.535 0.505 0.433
0.475
0.5ng/ml 0.302 0.289 0.217
0.275
0ng/ml 0.073 0.072 0
0.071

Precision

We measured random samples of E4033hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.8 0.21 3.6
Intra-Assay 2 18 3.4 0.18 5.4
Intra-Assay 3 18 0.9 0.05 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E4033hu

    $320.00

  • 96T

    E4033hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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