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Home > Product > ELISA Kit > Canine Von Willebrand Factor, VWF ELISA Kit

Canine Von Willebrand Factor, VWF ELISA Kit

Canine Von Willebrand Factor, VWF ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 96T,48T

Sensitivity: 0.15ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Von Willebrand Factor

Code

E0022Ca

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.15ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma (By similarity).

UniProt accession

MASS(Da)

309,719

GeneID

399544

Synonyms

von Willebrand factor;vWF

Gene names

VWF

Research area

Cardiovascular

Target protein

VWF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0022Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 1.826 1.871 1.809
1.916
24ng/ml 1.051 1.019 0.957
0.987
12ng/ml 0.531 0.527 0.465
0.523
6ng/ml 0.241 0.258 0.196
0.275
3ng/ml 0.072 0.1 0.038
0.128
0ng/ml 0.063 0.062 0
0.06

Precision

We measured random samples of E0022Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.6 0.46 4.3
Intra-Assay 2 18 38.7 1.46 3.8
Intra-Assay 3 18 20.2 0.72 3.6

Protocols

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  • Size

    Code

    Price

  • 96T

    E0022Ca

    $475.00

  • 48T

    E0022Ca

    $350.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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