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Home > Product > ELISA Kit > Human Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Human Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Human Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Calpain-1 Catalytic Subunit

Code

E4116hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed: 21531719, PubMed: 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed: 23707407).

UniProt accession

MASS(Da)

81,890

GeneID

823

Synonyms

Calcium-activated neutral proteinase 1;Calpain mu-type;Calpain-1 catalytic subunit;Calpain-1 large subunit;CANP 1;CAPN 1;CAPN1;Cell proliferation-inducing gene 30 protein;Micromolar-calpain;muCANP

Gene names

CAPN1

Research area

Others

Target protein

CAPN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4116hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.426 2.276 2.243
2.126
6ng/ml 1.416 1.379 1.346
1.342
3ng/ml 0.682 0.67 0.637
0.658
1.5ng/ml 0.359 0.33 0.297
0.3
0.75ng/ml 0.181 0.179 0.146
0.177
0ng/ml 0.047 0.033 0
0.019

Precision

We measured random samples of E4116hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.2 0.29 3.5
Intra-Assay 2 18 4.9 0.21 4.3
Intra-Assay 3 18 2.8 0.15 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E4116hu

    $320.00

  • 96T

    E4116hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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