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Home > Product > ELISA Kit > Human Merlin, NF2 ELISA Kit

Human Merlin, NF2 ELISA Kit

Human Merlin, NF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.29ng/L

Detection range: 15-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.29ng/L

Detection range: 15-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Merlin

Code

E4152hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.29ng/L

Detection range

15-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

UniProt accession

MASS(Da)

69,690

GeneID

4771

Synonyms

Merlin;Moesin-ezrin-radixin-like protein;Neurofibromin-2;NF 2;NF2;Schwannomerlin;Schwannomin

Gene names

NF2

Research area

Neuroscience

Target protein

NF2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4152hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.16 2.127 2.084
2.095
1000ng/L 1.089 1.03 0.986
0.971
500ng/L 0.49 0.51 0.467
0.531
250ng/L 0.184 0.23 0.186
0.276
125ng/L 0.159 0.159 0.116
0.16
0ng/L 0.058 0.044 0
0.029

Precision

We measured random samples of E4152hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1035.7 48.33 4.7
Intra-Assay 2 18 1223.2 32.96 2.7
Intra-Assay 3 18 382.4 19.21 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E4152hu

    $320.00

  • 96T

    E4152hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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