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Home > Product > ELISA Kit > Human Nadphoxidase 4, NOX4 ELISA Kit

Human Nadphoxidase 4, NOX4 ELISA Kit

Human Nadphoxidase 4, NOX4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.36ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.36ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nadph Oxidase 4

Code

E4156hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.36ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium Channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. [Isoform 4]: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.

UniProt accession

MASS(Da)

66,932

GeneID

50507

Synonyms

Kidney oxidase-1;Kidney superoxide-producing NADPH oxidase;KOX-1;NADPH oxidase 4;NOX 4;NOX4;Renal NAD(PH-oxidase

Gene names

NOX4

Research area

Cardiovascular

Target protein

NOX4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4156hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.376 2.241 2.189
2.106
400ng/L 1.229 1.288 1.236
1.347
200ng/L 0.767 0.771 0.719
0.775
100ng/L 0.306 0.347 0.295
0.387
50ng/L 0.158 0.172 0.12
0.187
0ng/L 0.051 0.052 0
0.053

Precision

We measured random samples of E4156hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 268.4 14.91 5.6
Intra-Assay 2 18 399.6 14.02 3.5
Intra-Assay 3 18 103.4 5.75 5.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E4156hu

    $320.00

  • 96T

    E4156hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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